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BCH00027 - OXIDATIVE STRESS IN THE HEART OF FAT INDUCED INSULIN RESISTANT RATS


ABSTRACT

                Insulin resistance was induced in Sprague Dawley rats by using a high fat diet (HFD) formula. Insulin resistance was induced in 10 weeks. The weight gained by rats fed HFD formula, was significantly more than those fed with normal diet. This study was aimed to investigate the level of oxidative stress in heart of fat induced insulin resistance rats. A total of twelve rats (n=6 per group i.e. experimental and control) was used for the study with each group fed with highly palatable high fat diet and normal standard feed. There was a significant increase in heart tissue levels of GSH in high fat rats as compared with the control group. Heart tissues also indicated levels of lipid peroxidation in high MDA concentration. Oral glucose tolerance test was carried out before the feeding programme and animals were sacrificed and the heart tissues were harvested. They were homogenized in PBS 0.01M on ice, centrifuged and taken for detection to assay levels reduced GSH, MDA. Data in the study are presented as mean±s.e.m and p-values less than 0.05 was taken to be statistically significant.

Lipid peroxidation in the heart leads to loss of the cellular membrane integrity due to oxidative modification of lipids and proteins that can ultimately lead to cardiac arrhythmias, poor contractility, infarction, cardiac failure or sudden death.