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ANP00009 - OCCURRENCE OF STAPHYLOCOCCUS AUREUS ON A FARM WITH SMALL SCALE PRODUCTION OF RAW MILK CHEESE


ABSTRACT

Staphylococcus aureus is an important pathogen that can cause Staphylococcal Food Poisoning (SFP). Milk and dairy products are frequently contaminated by this bacterium. In this study, 8 samples (4 raw milk and 4 dairy cheese) were collected from a small scale farm at Alomaja Orisunbare village in Oyo state, Nigeria, with the objective of detection and enumeration of S. aureus and other Staphylococcus species. Baird Parker agar for isolation and 16S rRNA gene sequencing for species identification were employed. S. aureus was found in 20% of sample from cow 3 with Total Aerobic Count of 1.1x104 CFUs/g. The prevalence of S. aureus was significantly greater in raw milk samples than in dairy products. Of all S. aureus positive samples, 20% contained >5 log10 CFU ml-1. Samples from small-scale dairies were twice more likely to be contaminated by S. aureus than traditionally managed dairies (P<0.05, OR=2.0). Coagulase-negative staphylococci (CNS) were found in 51.6% of the samples. The frequency of isolation found in this study indicates that S. aureus and other Staphylococcus spp. may impose public health hazard in dairy products. Therefore, further studies on the enterotoxigenic potential of the isolates, and molecular epidemiology to trace the sources of the contamination are recommended.